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DTE, Dithioerythritol can be used for maintaining thiol groups in a reduced state during protein processing, isolation, and denaturing.

EDAC, is a water-soluble carbodiimide derivative and can be used used to conjugate proteins and peptides.

PMSF is often used in lysis buffer to assist in preserving proteins of interest during protein isolation and sample preparation.

Guanidine hydrochloride has commonly been used for the isolation of RNA, to denature globular proteins and for protein refolding studies. It can also be used to facilitate the generation of tryptic peptides for analysis of complex protein samples.