Guanidine hydrochloride has commonly been used for the isolation of RNA, to denature globular proteins and for protein refolding studies. It can also be used to facilitate the generation of tryptic peptides for analysis of complex protein samples.

D-Galactose can be used in biopharmaceutical processes (both upstream and downstream).

Urea can be used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins.

Sucrose can be used in biopharmaceutical processes (both upstream and downstream).