PIPES can be used in a wide array of biological applications, including as a component in media formulations. Specific applications include buffers for cell culture and diagnostic reagent production.

DTE, Dithioerythritol can be used for maintaining thiol groups in a reduced state during protein processing, isolation, and denaturing.

EDAC, is a water-soluble carbodiimide derivative and can be used used to conjugate proteins and peptides.

DHAA, Dehydroascorbic acid can be used in the oxidation of proteins.