Guanidine Thiocyanate has commonly been used for the isolation of RNA, to denature globular proteins and for protein refolding studies. It can also be used to facilitate the generation of tryptic peptides for analysis of complex protein samples.

Tris HCl can be used in biopharmaceutical buffer formulations (both upstream and downstream), electrophoresis separations and diagnostic reagent formulations. Tris HCl is especially useful in downstream purification processes.

DTE, Dithioerythritol can be used for maintaining thiol groups in a reduced state during protein processing, isolation, and denaturing.

EDAC, is a water-soluble carbodiimide derivative and can be used used to conjugate proteins and peptides.